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KMID : 1020220230230040209
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Journal of Korean Society of Dental Hygiene
2023 Volume.23 No. 4 p.209 ~ p.218
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Effects of antibacterial mouth rinses on multiple oral biofilms model
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Jun Soo-Kyung
Choi Young-Suk
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Abstract
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Objectives: To confirm the antibacterial effects of each mouth rinse on multiple oral biofilms in vitro.
Methods: The antibacterial effects of different mouth rinses were examined by ATP and counted colony forming units (CFU). Preformed oral biofilms on saliva coated hydroxyapatite (sHA) disks were treated with essential oil and saline; then, the multiple oral biofilms were observed by Scanning electron microscope (SEM). RNA sequencing analysis was performed on total RNA isolated from old biofilms of P. intermedia ATCC 49046.
Results: In the CFU measured result compared to controls, preformed multiple oral biofilms were reduced from a low of 39.0% to 95.7% (p <0.05). The size of bacterial cells changed after treatment with the essential oil, and some of the cells ruptured into small pieces of cell debris. Gene expression in P. intermedia ATCC 49046 significantly altered in RNA transcribed and protein translated genes after exposure to essential oil.
Conclusions: Mouth rinse solutions with different ingredients had different antibacterial effects and may alter surface structure and gene expression as determined by RNA sequencing.
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KEYWORD
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Essential oil, Mouth rinse, Oral biofilms, RNA sequence
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